Effect of Low Night Temperatures on Photosynthetic Activity of the Maize Seedlings (Zea mays L.)

Night chilling (5 °C) subsequently lowered photosynthetic intensity in the leaves of maize seedlings at 20 °C through an increase in leaf diffusive resistance brought on by lower tissue water content in morning hours. A more significant increase in leaf diffusion resistance was observed when soil temperature was lowered than in the case of lower air temperature.
The unfavorable effect of soil and air cooling temperature on photosynthesis was limited by air saturated with water vapour. However, as a result of lowering the night temperature from 5 °C to 1 °C, the efficiency of the protective influence of higher atmospheric humidity was decreased. This demonstrates that the participation of factors unrelated to plant water status in inhibiting photosynthesis increases with lower night temperatures.
An additional reason for inhibited photosynthesis following cool nights was a decrease in chlorophyll accumulation, below 50 μg per 1 cm² of leaf area.     

大豆室内耐冷筛选及其在田间的应用研究

本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。     

大蒜不定芽的诱导及其增殖系数的调节

为获得白皮大蒜组培繁殖体系,以其茎尖为材料,通过BA和NAA不同激素配比试验研究,结果表明:诱导愈伤组织的最适培养基为:MS BA0.2mg/L NAA0.5mg/L,诱导率达60%;诱导不定芽的最适培养基为:MS BA1.0mg/L NAA0.1mg/L,丛生芽繁殖系数高达80%。同时,为建立扩繁最佳体系,分别研究了激素、pH、谷氨酸钠三因素对不定芽增殖系数的调节效果,研究表明:添加0.5mg/L的GA3使增值系数提高到13.8;pH5.8最利于不定芽增值;添加10mg/L谷氨酸钠能在多次继代培养中保持较高的增值系数。     

胡萝卜黄色突变体的遗传及表现研究

 从胡萝卜‘新黑田五寸’品种的高世代自交系中发现了能稳定遗传的黄色突变体。遗传分析证明该黄色突变性状为单基因隐性遗传, 绿色对黄色为完全显性。黄色突变体叶片中叶绿素含量仅为正常株的29.3% ～50.1% , 且黄色不受温度光照等环境的影响。黄色突变体( yelyel) 地上部和地下部的生长量明显少于正常株系( YELYEL ) , 但在等位基因杂合时( YELyel) 与正常株系的生长量、产量和营养品质无显著差异。     

笃斯越桔硬枝扦插繁殖技术研究

采用笃斯越桔硬枝扦插,用生根剂处理插穗,并在不同的基质上进行扦插试验,结果表明:笃斯越桔硬枝扦插用河沙作为基质最好,生根剂ABT处理的插穗生根率最高。     

新型的人工饲料防腐剂ADA

将新型防腐剂——ＡＤＡ添入家蚕人工饲料中，对在高温多湿养蚕环境下，易引起人工饲料霉变的酵母菌群、格兰氏阴性菌群和霉菌群有明显的抑制作用，可延长人工饲料的存放和使用时间，而不影响其适口性、颜色、香味与成份，防腐效果明显优于苯甲酸、山梨酸、脱氢乙酸、丙酸等．实验证明：人工饲料中添加０．２％的ＡＤＡ能使人工饲料在高温多湿的养蚕环境下保持一个月不发霉变质，并对蚕体的生长发育无不良影响     

狗舌草提取物诱导淋巴细胞性白血病L1210细胞分化的研究

以单猪屎豆碱（MO）、槲皮素（QU）和环磷酰胺（CY）为参照，观察了狗舌草600mL／L乙醇提取物（EX）对淋巴细胞性白血病L1210细胞体外试验的形态变化；利用流式细胞术，从DNA分子水平上检查了EX对L1210细胞各周期相的影响，探讨EX对L1210细胞的分化机理。结果发现，EX能够使L1210细胞向淋巴细胞方向发展；经EX作用24h后，L1210细胞G0＋G1期的百分比较对照组明显升高。提示EX对L1210细胞增殖的抑制作用可能是由于G1期的阻滞所致。     

影响烟草花粉胚胎发生的某些因子——铁盐、蔗糖和腺素

本文研究了铁盐、蔗糖等培养基成份对烟草花粉胚胎形成的影响。结果表明,铁盐和蔗糖与烟草花粉胚形成有密切关系。培养基中没有铁盐,胚发育停止球形期;没有蔗糖、胚发育受阻在多细胞原胚期,不能进一步发育。培养基中仅有蔗糖,胚发育停止在鱼雷胚早期,将其转到只含铁盐的培养基中,鱼雷胚可继续发育直至成株。培养基中不附加铁盐或蔗糖,胚的发育均受阻。只有铁盐和蔗糖而无大量元素、微量元素及有机成份,即能形成胚状体并再生植株,但胚胎发生频率低,表明其它附加成份并不是没有意义的。另外,培养基中附加一定浓度的腺素对花粉胚形成是有利的,但高浓度是有害的。     

一种微生态制剂中抑菌物质的分离与鉴定

植物乳杆菌发酵产生的抑菌物质经乙醇浸提、SephadexG-15层析、氨基酸组成分析、反相液相色谱分离及鉴定，发现该植物乳杆菌产生的抑菌物质为一类分子量较小（小于1000Da)，且比较接近，极性不同的多肽。     

Effect of Split Nitrogen Application on Growth and Yield of Wheat (T. aestivum L.) Genotypes with Different N-Assimilation Potential

A field experiment was conducted with different nitrogen regimes to assess the growth and yield performance of wheat genotypes which differ in nitrate assimilation potential. Genotypic differences in biomass accumulation were observed at different growth stages. The nitrogen treatment had little effect on biomass accumulation at early stages of growth, while at later stages of growth there was enhanced biomass accumulation when N was applied in more than two splits. On an average, genotypes with high nitrate reductase activity (the 'HNR' genotypes) accumulated 14.2 % more biomass than the genotypes with low nitrate reductase activity ('LNR' genotypes) when an extra dose (40 kg N ha⁻¹) of nitrogen was given at the time of anthesis. The application of nitrogen in more than two splits increased grain yield of both 'HNR' and 'LNR' genotypes mainly by increasing grain weight per ear. The application of an extra dose of nitrogen (40 kg N ha⁻¹) at the time of anthesis increased grain yield of 'HNR' genotypes by 38.5 % as compared to 'LNR' genotypes.